Interleukin 10 （IL-10），originally known as cytokine synthesis inhibitory factor （CSIF），is a pleiotropic cytokine with a myriad of immunomodulatory effects on a variety of cell types. On activated B cells，IL-10 can induce the formation of plasma cells and the secretion of IgG or，in the presence of TGF-beta1 and/or IL-4，the secretion of IgA. On macrophages，IL-10 is known to downregulate IL-1，TNF-alpha and IL-6 production. On dendritic cells，IL-10 has been shown to interfere with antigen-presenting cell （APC） functions by downmodulating the expression of stimulatory and co-stimulatory molecules. IL-10 has also been found to inhibit dendritic cell differentiation from monocytes and to promote monocyte maturation into cytotoxic CD16+ macrophages. Rat IL-10 cDNA encodes a 178 aa residue precursor protein that contains an 18aa signal sequence and a 160aa mature protein. Mature rat IL-10 shares 85% and 74%aa sequence identity with mouse and human IL-10，respectively.Sample Type：Cell culture supernatants，serum，plasma，and tissueIntended Use：The Rat IL-10 BioAssay ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative detection of Rat IL-10 concentrations in cell culture supernatants，serum，plasma，and tissue.Sensitivity：30pg/mlRange：62.5-4000pg/mlSpecificity：Recognizes rat IL-10.Sample Volume：100ul/wellTest Principle：This assay employs the Sandwich immunoassay technique. An anti-r IL-10 monoclonal coating antibody is adsorbed onto microwells. IL-10 present in the sample or standard and Biotinylated anti-IL-10 antibody binds to antibodies adsorbed to the microwells. Following incubation unbound sample or standard and Biotinylated anti-IL-10 antibody are removed during a wash step. A Streptavidin-HRP is added and binds to Biotinylated anti-IL-10 antibody. Following incubation unbound Streptavidin-HRP is removed during a wash step. A colored product is formed in proportion to the amount of IL-10 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-10 standard dilutions and IL-10 sample concentration determined.Kit Components：Aluminium pouches with a Microwell Plate： 8x12Standard： 2xVialBiotinylated antibody： 2xVialStreptavidin-HRP： 2xVialAssay Buffer （25ml/Vial）： 2xVialWash Buffer Concentrate 20x （30ml/Vial）： 1xVialTMB Substrate Solution： 1x12mlStop Solution （12ml/Vial）： 1xVialPlate Covers- Adhesive strips： 3xstripsInstruction： 1xStorage and Stability：Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product，centrifuge the original vial after thawing and prior to removing the cap.
