Interleukin 4 （IL-4） is a pleiotropic cytokine produced by activated T cells，mast cells，and basophils. It was initially identified as a B cell differentiation factor （BCDF），as well as a B cell stimulatory factor （BSF1）. IL-4 has since been shown to have multiple biological effects on hematopoietic and non-hematopoietic cells，including B and T cells，monocytes，macrophages，mast cells，myeloid and erythroid progenitors，fibroblasts，and endothelial cells. Rat，mouse and human IL-4 are species-specific in their activities.Sample Type：Cell culture supernatants，serum，plasma，and tissueIntended Use：The Rat IL-4 BioAssay ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative detection of Rat IL-4 concentrations in cell culture supernatants，serum，plasma，and tissue.Sensitivity：15pg/mlRange：31.25-2000pg/mlSpecificity：Recognizes rat IL-4.Sample Volume：100ul/wellTest Principle：This assay employs the Sandwich immunoassay technique. An anti-r IL-4 monoclonal coating antibody is adsorbed onto microwells. IL-4 present in the sample or standard and Biotinylated anti-IL-4 antibody binds to antibodies adsorbed to the microwells. Following incubation unbound sample or standard and Biotinylated anti-IL-4 antibody are removed during a wash step. A Streptavidin-HRP is added and binds to Biotinylated anti-IL-4 antibody. Following incubation unbound Streptavidin-HRP is removed during a wash step. A colored product is formed in proportion to the amount of IL-4 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-4 standard dilutions and IL-4 sample concentration determined.Kit Components：Aluminium pouches with a Microwell Plate： 8x6Standard： 1xVialBiotinylated antibody： 1xVialStreptavidin-HRP： 1xVialAssay Buffer （25ml/Vial）： 1xVialWash Buffer Concentrate 20x （30ml/Vial）： 1xVialTMB Substrate Solution： 1x6mlStop Solution （12ml/Vial）： 1xVialPlate Covers- Adhesive strips： 2xstripsInstruction： 1xStorage and Stability：Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product，centrifuge the original vial after thawing and prior to removing the cap.
