- 中文名称
IL-4 BioAssay ELISA Kit (Mouse) (Interleukin-4，IL-4，B-cell stimulatory factor 1，BSF-1，Binetrakin，Lymphocyte stimulatory factor 1，Pitrakinra，IL4)
- 英文名字
- IL-4 BioAssay ELISA Kit(Mouse)(Interleukin-4，IL-4，B-cell stimulatory factor 1，BSF-1，Binetrakin，Lymphocyte stimulatory factor 1，Pitrakinra，IL4)
- 供应商
- USBiological
- 产品货号
- 360516
- 产品报价
- ￥1/96Tests
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Interleukin 4 （IL-4） is a pleiotropic cytokine produced primarily by activated T lymphocytes，mast cells and basophils. IL-4 has multiple immune response-modulating activities on a variety of cell types. It is an important regulator of isotype switching，inducing IgE production in B lymphocytes. It is an important modulator of the differentiation of precursor T helper cells to the Th2 subset that mediates humoral immunity and modulates antibody production. In addition，IL-4 has also been shown to have anti-tumor activity both in vivo and in vitro. The cDNA sequence of mouse IL-4 predicts a 140aa residue precursor protein containing a 20aa residue signal peptide that is cleaved to form the mature protein. At the amino acid sequence level，mature mouse IL-4 is approximately 50% identical to human IL-4 but there is no species cross-reactivity for biological activity for the two proteins.Sample Type：Cell culture supernatants，serum，plasma，and tissueIntended Use：The Mouse IL-4 BioAssay ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative detection of Mouse IL-4 concentrations in cell culture supernatants，serum，plasma，and tissue.Sensitivity：7pg/mlRange：15.625-1000pg/mlSpecificity：Recognizes mouse IL-4. There was no detectable cross reactivity with any of the tested proteins following IL-1,2,3,5,6,10，8,12,TNF,VEGF,TGF and IFN.Sample Volume：100ul/wellTest Principle：This assay employs the Sandwich immunoassay technique. An anti-m IL-4 monoclonal coating antibody is adsorbed onto microwells. IL-4 present in the sample or standard and Biotinylated anti IL-4 antibody binds to antibodies adsorbed to the microwells. Following incubation unbound sample or standard and Biotinylated anti IL-4 antibody are removed during a wash step. A Streptavidin-HRP is added and binds to Biotinylated anti IL-4 antibody. Following incubation unbound Streptavidin-HRP is removed during a wash step. A colored product is formed in proportion to the amount of IL-4 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-4 standard dilutions and IL-4 sample concentration determined.Kit Components：Aluminium pouches with a Microwell Plate： 8x12Standard： 2xVialBiotinylated antibody： 2xVialStreptavidin-HRP： 2xVialAssay Buffer （25ml/Vial）： 2xVialWash Buffer Concentrate 20x （30ml/Vial）： 1xVialTMB Substrate Solution： 1x12mlStop Solution （12ml/Vial）： 1xVialPlate Covers- Adhesive strips： 3xstripsInstruction： 1xStorage and Stability：Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product，centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对IL-4 BioAssay ELISA Kit (Mouse) (Interleukin-4，IL-4，B-cell stimulatory factor 1，BSF-1，Binetrakin，Lymphocyte stimulatory factor 1，Pitrakinra，IL4)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到IL-4 BioAssay ELISA Kit （Mouse） （Interleukin-4，IL-4，B-cell stimulatory factor 1，BSF-1，Binetrakin，Lymphocyte stimulatory factor 1，Pitrakinra，IL4）产品后将其置于4°C至-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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