Interleukin 2 （IL-2） was initially identified as a T cell growth factor that is produced by T cells following activation by mitogens or antigens. Since then，it has also been shown to stimulate the growth and differentiation of B cells，natural killer （NK） cells，lymphocyte activated killer （LAK） cells，monocytes/macrophages and oligodendrocytes. At the amino acid sequence level，there is approximately 60% - 90% similarity between species. Mature human IL-2 shows 65%，67%，72%，78%，and 64%aa identity to mouse，rat，porcine，feline，and bovine IL-2，respectively.Sample Type：Cell culture supernatants，serum，plasma，and tissueIntended Use：The Rat IL-2 BioAssay ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative detection of Rat IL-2 concentrations in cell culture supernatants，serum，plasma，and tissue.Sensitivity：15pg/mlRange：15.625-1000pg/mlSpecificity：Recognizes rat IL-2.Sample Volume：100ul/wellTest Principle：This assay employs the Sandwich immunoassay technique. An anti-r IL-2 monoclonal coating antibody is adsorbed onto microwells. IL-2 present in the sample or standard and Biotinylated anti-IL-2 antibody binds to antibodies adsorbed to the microwells. Following incubation unbound sample or standard and Biotinylated anti-IL-2 antibody are removed during a wash step. A Streptavidin-HRP is added and binds to Biotinylated anti-IL-2 antibody. Following incubation unbound Streptavidin-HRP is removed during a wash step. A colored product is formed in proportion to the amount of IL-2 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-2 standard dilutions and IL-2 sample concentration determined.Kit Components：Aluminium pouches with a Microwell Plate： 8x12Standard： 2xVialBiotinylated antibody： 2xVialStreptavidin-HRP： 2xVialAssay Buffer （25ml/Vial）： 2xVialWash Buffer Concentrate 20x （30ml/Vial）： 1xVialTMB Substrate Solution： 1x12mlStop Solution （12ml/Vial）： 1xVialPlate Covers- Adhesive strips： 3xstripsInstruction： 1xStorage and Stability：Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product，centrifuge the original vial after thawing and prior to removing the cap.
