Interleukin 1 （IL-1） includes two distinct proteins，IL-1alpha and IL-1beta，that play central roles in acute and chronic inflammation，both locally and systemically. Human IL-1beta is synthesized as a 269aa，31kD precursor protein （prepro-IL-1beta） that is cleaved by IL-1beta-converting enzyme （ICE） to the 153aa，17kD mature IL-1beta plus a prosegment. Some combination of the mature form，the prosegment and prepro-IL-1betais released from the cell. IL-1beta is produced primarily by monocytes and macrophages but also by astrocytes，oligodendroglia，adrenal cortical cells，NK cells，endothelial cells，keratinocytes，megakaryocytes，platelets，neurons，neutrophils，osteoblasts，Schwann cells，trophoblasts，T cells，and fibroblasts. The most extensively studied function of IL-1 is initiation of inflammation. Bacterial endotoxin or a variety of non-microbial inflammatory substances induce production of IL-1，which is released into the local environment. IL-1 induces capillary endothelial cells to secrete chemokines （e.g. MCP-1） and to increase expression of cell adhesion molecules （e.g. E-selectin，ICAM-1 and VCAM-1）.Sample Type：Cell culture supernatants，serum，plasma，and tissueIntended Use：The Human IL-1beta BioAssay ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative detection of Human IL-1beta concentrations in cell culture supernatants，serum，plasma，and tissue. Sensitivity：4pg/mlRange：7.8-500pg/mlSpecificity：Recognizes human IL-1beta.Test Principle：This BioAssay employs the Sandwich immunoassay technique. A human IL-1beta monoclonal coating antibody is adsorbed onto microwells. IL-1beta present in the sample or standard binds to antibodies adsorbed to the microwells. Following incubation unbound sample or standard are removed during a wash step. a Biotinylated IL-1beta antibody is added and binds to IL-1beta captured by the first antibody. Following incubation unbound Biotinylated IL-1beta antibody is removed during a wash step. A Streptavidin-HRP is added and binds to Biotinylated anti-h IL-1beta antibody. Following incubation unbound Streptavidin-HRP is removed during a wash step. A colored product is formed in proportion to the amount of IL-1beta present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-1beta standard dilutions and IL-1beta sample concentration determined.Kit Components：Aluminium pouches with a Microwell Plate，8x12Standard，2x vialsBiotinylated antibody，2x vialsStreptavidin-HRP，2x vialsAssay Buffer，2x 25mlWash Buffer Concentrate 20x，1x30mlTMB Substrate Solution，1x12mlStop Solution，1x12mlPlate Covers- Adhesive strips，3x stripsInstruction，1x manualStorage and Stability：Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product，centrifuge the original vial after thawing and prior to removing the cap.
