Interleukin 18 （IL-18），also known as interferon-gamma-inducing factor （IGIF） and IL-1gamma，is a cytokine which shares biologic activities with IL-12 and structural similarities with the IL-1 family of proteins. IL-18 was originally cloned from liver cells and has since been shown to be expressed by monocyte/macrophages，osteoblasts and keratinocytes. Caspase-1 （IL-1 beta-converting enzyme） has been implicated in the physiological processing of pro-IL-18 to IL-18. Similarly to IL-12，human IL-18 has been shown to enhance NK cell activity in PBMC cultures. Human IL-18 has also been found to induce the production IFN-gamma and GM-CSF while inhibiting the production of IL-10 by PBMC. On enriched human T cells，human IL-18 can enhance Th1 cytokine production and stimulate cell proliferation via an IL-2-dependent pathway. In the mouse system，IL-18 has been shown to be a costimulatory factor for the activation of Th1，but not Th2，cells. IL-18 was found to selectively enhance the FasL-mediated cytotoxicity of Th1，but not Th0 or Th2，cells. IL-18 has also been shown to induce activated B cells to produce IFN-gamma that inhibits IgE production.Sample Type：Cell culture supernatants，serum，plasma，and tissueIntended Use：The Human IL-18 BioAssay ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative detection of Human IL-18 concentrations in cell culture supernatants，serum，plasma，and tissue.Sensitivity：4pg/mlRange：1000-15.625pg/mlSpecificity：Recognizes human IL-18.Sample Volume：100ul/wellTest Principle：This assay employs the Sandwich immunoassay technique. An anti-h IL-18 monoclonal coating antibody is adsorbed onto microwells. IL-18 present in the sample or standard binds to antibodies adsorbed to the microwells. Following incubation unbound sample or standard are removed during a wash step. a Biotinylated anti-h IL-18 antibody is added and binds to IL-18 captured by the first antibody. Following incubation unbound Biotinylated anti-h IL-18 antibody is removed during a wash step. A Streptavidin-HRP is added and binds to Biotinylated anti-h IL-18 antibody. Following incubation unbound Streptavidin-HRP is removed during a wash step. A colored product is formed in proportion to the amount of IL-18 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-18 standard dilutions and IL-18 sample concentration determined.Kit Components：Aluminium pouches with a Microwell Plate： 8x6Standard： 1xVialBiotinylated antibody： 1xVialStreptavidin-HRP： 1xVialAssay Buffer （25ml/Vial）： 1xVialWash Buffer Concentrate 20x （30ml/Vial）： 1xVialTMB Substrate Solution： 1x6mlStop Solution （12ml/Vial）： 1xVialPlate Covers- Adhesive strips： 2xstripsInstruction： 1xStorage and Stability：Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product，centrifuge the original vial after thawing and prior to removing the cap.
