Interleukin 11 （IL-11），also known as adipogenesis inhibitory factor （AGIF），has multiple effects on both hematopoietic and nonhematopoietic cells. Many of the biological effects described for IL-11 overlap those for IL-6. IL-11 exerts its biological activities through binding to a specific high-affinity receptor complex consisting of an IL-11 receptor chain and gp130.Sample Type：Cell culture supernatants，serum，plasma，and tissueIntended Use：The Mouse IL-11 BioAssay ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative detection of Mouse IL-11 concentrations in cell culture supernatants，serum，plasma，and tissue.Sensitivity：15pg/mlRange：31.25-2000pg/mlSpecificity：Recognizes mouse IL-11.Sample Volume：100ul/wellTest Principle：This assay employs the Sandwich immunoassay technique. An anti-m IL-11 monoclonal coating antibody is adsorbed onto microwells. IL-11 present in the sample or standard binds to antibodies adsorbed to the microwells. Following incubation unbound sample or standard are removed during a wash step. a Biotinylated anti-m IL-11 antibody is added and binds to IL-11 captured by the first antibody. Following incubation unbound Biotinylated anti-m IL-11 antibody is removed during a wash step. A Streptavidin-HRP is added and binds to Biotinylated anti-m IL-11 antibody. Following incubation unbound Streptavidin-HRP is removed during a wash step. A colored product is formed in proportion to the amount of IL-11 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-11 standard dilutions and IL-11 sample concentration determined.Kit Components：Aluminium pouches with a Microwell Plate： 8x6Standard： 1xVialBiotinylated antibody： 1xVialStreptavidin-HRP： 1xVialAssay Buffer （25ml/Vial）： 1xVialWash Buffer Concentrate 20x （30ml/Vial）： 1xVialTMB Substrate Solution： 1x6mlStop Solution （12ml/Vial）： 1xVialPlate Covers- Adhesive strips： 2xstripsInstruction： 1xStorage and Stability：Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product，centrifuge the original vial after thawing and prior to removing the cap.
