Aromatase is a key enzyme in steroidogenesis and plays an important role in sexual differentiation,fertility,and carcinogenesis. Many environmental chemicals may influence aromatase activity and thereby disrupt endocrine function.
应用类型
Western blotting (0.5-4 ug/ml), Immunohistochemistry (10-20 ug/ml). However, the optimal concentrations should be determined individually. Jurkat cell lysate can be used as a positive control. The antibody should recognize aromatase in samples from human, mouse, rat, bovine, porcine, pig, sheep, rabbit, horse and chicken origins. A 55 kDa band can be detected, corresponding to the expected molecular weight of Aromatase. An additional 35 kDa band (believe to be the cleavage product of Aromatase) can also be detected in Jurkat cells.
免疫原
Synthetic peptide surrounding amino acid 385 of human Aromatase
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠、牛、鸡、猪、兔、马种属来源的Aromatase蛋白发生免疫反应.
保存建议
提供的Aromatase抗体产品为100 ug 无色溶液,其浓度为0.2 mg/ml,溶解于包含有30%甘油, 0.5% BSA和0.01%硫柳汞的PBS缓冲液中.请将该Aromatase抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.