Due to the various splice variants，the trk proteins exist as variably glycosylated entities with the major forms having molecular weights of 140-145 kDa，110 kDa and the unglycosylated form of 80 kDa. trkB contains 33.3% carbohydrate by weight representing modification on 10 of 12 N-glycosylation sites. The primary ligand for trkB is BDNF which induces the phosphorylation of the protein and subsequent binding of PLC-gamma via SH2 domains. TrkB may function to modulate neuronal responses to the neurotrophins acting throμgh trkB such as BDNF.
应用类型
Western blot, Immunoprecipitation, Immunofluorescence
免疫原
Synthetic peptide surrounding amino acid 810 of human TrkB
