Due to the various splice variants，the trk proteins exist as variably glycosylated entities with the major forms having molecular weights of 140-145 kDa，110 kDa and the unglycosylated form of 80 kDa. trkB contains 33.3% carbohydrate by weight representing modification on 10 of 12 N-glycosylation sites. The primary ligand for trkB is BDNF which induces the phosphorylation of the protein and subsequent binding of PLC-gamma via SH2 domains. TrkB may function to modulate neuronal responses to the neurotrophins acting through trkB such as BDNF.
应用类型
Western blotting （0.5-4 ug/ml）， immunofluorescence （5-10 ug/ml）. However， the optimal concentrations should be determined individually. Trk protein exists as variably glycosylated entities with the major forms having molecular weights of 140 kDa， 110 kDa， and the unglycosylated form of 80 kDa.
免疫原
Synthetic peptide surrounding amino acid 810 of human TrkB
来源宿主
兔
反应性
经检测验证，可与人、小鼠、大鼠、鸡种属来源的TrkB蛋白发生免疫反应.
保存建议
提供的TrkB抗体产品为100 ug 无色溶液，其浓度为0.2 mg/ml，溶解于包含有30%甘油， 0.5% BSA和0.01%硫柳汞的PBS缓冲液中.请将该TrkB抗体产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
