The JNK pathway is activated by largely distinct stimuli including inflammatory cytokines （such as TNF- and IL-1），UV light，inhibitors of protein synthesis and osmotic stress. Activated MEKK1 phosphorylates SEK1 （also known as MKK4），which in turn activates SAPK （also known as JNK）. JNK binds tightly to the N-terminal region of c-Jun and ATF-2，and phosphorylates c-Jun at Ser63 and Ser73 and ATF-2 at Thr69 and Thr71.
应用类型
Western blotting （1-2 ug/ml）， immunoprecipitation （10-20 ug/ml）， immunocytochemistry （10-20 ug/ml）， and Immunohistochemistry （20-30 ug/ml）. However， the optimal conditions should be determined individually. The antibody detects the p46 and p54 JNK/SAPK dually phosphorylated at Thr183 and Tyr185.
免疫原
Synthetic phospho-peptide corresponding to residues surrounding Thr183/Tyr185 of human SAPK/JNK
