LIM kinases （LIMK1 and LIMK2） are serine/threnine kinases that have two zinc finger motifs，known as LIM motifs in their N-terminal regulatory domain. LIM kinases are involved in actin cytoskeleton regulation through Rho-family GTPases and downstream kinases PAKs and ROCK. PAK1 and ROCK phosphorylate LIMK1 and LIMK2，which increases the activity of the kinases. Activated LIM kinases inhibit the actin depolymerization activity of cofilin by phosphorylation at the N-terminus of Cofilin.
应用类型
Western blotting （1-4 ug/ml）. However， the optimal conditions should be determined individually. The antibody detects 70 kDa LIMK2 protein. It does not cross-react with LIMK1.
免疫原
Synthetic peptide corresponding to the C-terminus of human LIMK2
