The Protein Kinase C （PKC） BioAssay ELISA Kit is a quantitative sandwich assay for the detection of PKC in rat serum，plasma，tissue homogenates and other biological fluids.Detection Range：15.625-1000pg/mlSensitivity：<9.375pg/mlPrecision： Intra-Assay CV： <8%Inter-Assay CV： <10%Assay Principle：The microtiter plate provided in this kit has been pre-coated with an antibody specific to PKC. Standards and samples are added to the appropriate microtiter plate wells followed by a biotin-conjugated antibody specific to PKC. Streptavidin conjugated to Horseradish Peroxidase （HRP） is added to each microplate well and incubated. After TMB substrate solution is added，only those wells that contain PKC，biotin-conjugated antibody and enzyme-conjugated streptavidin complex will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PKC in the sample is then determined by comparing the O.D. of the sample to the standard curve.Kit Components：*358152A： Microtiter Strips，1x96 wells （8x12 wells）*358152B： Standard，2x1 vial358152C： Sample/Standard Dilution Buffer，1x20ml358152D： Antibody-Biotin （Concentrated），1x120ul358152E： Antibody Dilution Buffer，1x10ml*358152F： Streptavidin-HRP Conjugate （SABC），1x120ul358152G： SABC Dilution Buffer，1x10ml*358152H： TMB Substrate，1x10ml358152J： Stop Solution，1x10ml358152K： Wash Buffer，25X，1x30mlStorage and Stability：Store unopened *358152A at 4ºC; store at -20ºC once opened. Store unopened *358152B at 4°C; once reconstituted store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store *358152F and *358152H at 4°C; protect from light. Store all other components at 4°C. Kit is stable for 6 months after receipt. For maximum recovery of product，centrifuge the original vials after thawing and prior to removing the cap.Assay Summary：1. Wash plate 2 times before adding standards，samples and control （zero） to wells.2. Add 100ul standard or sample to each well and incubate for 90 minutes at 37°C. Aspirate and wash 2 times.3. Add 100ul Antibody-Biotin working solution to each well and incubate for 60 minutes at 37°C4. Aspirate and wash 3 times.5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37°C6. Aspirate and wash 5 times.7. Add 90ul TMB substrate. Incubate 15-30 minutes at 37°C8. Add 50ul Stop Solution. Read at 450nm immediately.9. Calculate results.
产品特点
针对Protein Kinase C (PKC) BioAssay ELISA Kit (Rat)该产品的特点优势，欢迎查阅官网提供的产品说明书。
保存建议
建议收到Protein Kinase C （PKC） BioAssay ELISA Kit （Rat）产品后将其置于4°C至-20°C保存。
