Members of Oct family of transcription factors specifically interact with Octamer motif ATGCAAAT，a regulatory element important for tissue- and cell-specific transcription as well as for transcription of a number of housekeeping genes. All of the members of the Oct family contain two highly conserved domains which are separated by 14-26 variable amino acids. These include the POU homeodomain and the POU-specific domain. Both are required for DNA binding and are involved in protein-protein interactions. Evidences indicate that regulation of Oct family transcription factors occurs at the level of phosphorylation.
应用类型
Western blotting （0.5-4 ug/ml）， immunoprecipitation （20 ug/ml） and Immunohistochemistry （20 ug/ml）. However， the optimal conditions should be determined individually. The antibody reacts with 45 kDa Oct-4 （also designated Oct-3A） and in a less extent 33 kDa Oct-3B from human， mouse， and rat samples. Rat kidney tissue lysate can be used as a positive control.
免疫原
Synthetic peptide mapping to the N-terminus of mouse Oct-4
