Wnt gene family members，including Wnt-1 through Wnt-10，play a key role in regulating cellular growth and differentiation. Wnt-1 is a cysteine-rich，secreted glycoprotein that associates with cell membranes and likely functions as a key regulator of cellular adhesion. Wnt-1，which is essential for normal development of the embryonic nervous system，contributes to hyperplasia and tumorigenic progression when improperly expressed in mammary tissue. Wnt-3 is involved in tumorigenesis and Wnt-2 and Wnt-4 may be associated with abnormal proliferation in human breast tissue. Wnt-6 is involved in oncogenesis and is co-expressed with Wnt10 in colorectal cancer cell lines. The presence of cervical and breast cell carcinomas may indicate an over expression of Wnt-6.
应用类型
Western blotting （0.5-4 ug/ml）， Immunohistochemistry （10-20 ug/ml）. However， the optimal concentrations should be determined individually. The antibody recognizes 40-46 kDa Wnt-6 from rat origins. Reactivity to other species has not been tested.
免疫原
Synthetic peptide surrounding amino acid 276 of mouse Wnt-6
来源宿主
兔
反应性
经检测验证，可与人、小鼠、大鼠种属来源的Wnt-6蛋白 发生免疫反应.
保存建议
提供的Wnt-6抗体 产品为100 ug 无色溶液，其浓度为0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA和0.01%硫柳汞nn的PBS缓冲液中.请将该Wnt-6抗体 产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
