- 中文名称
IL-12 (Interleukin 12) BioAssay ELISA Kit (Human)
- 英文名字
- IL-12 (Interleukin 12) BioAssay ELISA Kit (Human)
- 供应商
- USBiological
- 产品货号
- 356618
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- IL-12 (Interleukin 12) BioAssay ELISA Kit is for the quantitative detection of IL-12 in human serum, plasma, tissue homogenates and other biological fluids.Range:15.625-1000pg/mlSensitivity:<9.375pg/mlPrecision:Intra-assay CV: <8%Inter-assay CV: <10%Assay Principle:This kit was based on sandwich enzyme-linked immune-sorbent assay technology. IL-12 antibody was pre-coated onto 96-well plates. And the biotin conjugated IL-12 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrate was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-10 amount of sample captured on plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL-12 can be calculated.Kit Components:356618A: Microtiter Strips, 1x96 wells 356618B: Standard, 2x1vial356618C: Sample/Standard Dilution Buffer, 1x20ml356618D: Antibody (Biotin) (Concentrated), 1x120ul356618E: Antibody Dilution Buffer, 1x10ml356618F: Streptavidin (HRP) (SABC), 1x120ul356618G: SABC Dilution Buffer, 1x10ml356618H: TMB Substrate, 1x10ml356618J: Stop Solution, 1x10ml356618K: Wash Buffer, 25X, 1x30mlStorage and Stability:Store unopened *356618A at 4ºC. Once opened, store at -20ºC. Store *356618B powder at 4°C. Once reconstituted store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.Assay Summary:1. Wash plate 2 times before adding standards, samples and control (zero) to wells!2. Add 100ul standard or sample to each well for 90 minutes at 37°C3. Add 100ul Biotin antibody working solution to each well for 60 minutes at 37°C4. Aspirate and wash 3 times5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37°C6. Aspirate and wash 5 times7. Add 90ul TMB substrate. Incubate 10-20 minutes at 37°C8. Add 50ul Stop Solution. Read at 450nm immediately.9. Calculation of results
- 产品特点
- IL-12 (Interleukin 12) BioAssay ELISA Kit is for the quantitative detection of IL-12 in human serum, plasma, tissue homogenates and other biological fluids.Range:15.625-1000pg/mlSensitivity:<9.375pg/mlPrecision:Intra-assay CV: <8%Inter-assay CV: <10%Assay Principle:This kit was based on sandwich enzyme-linked immune-sorbent assay technology. IL-12 antibody was pre-coated onto 96-well plates. And the biotin conjugated IL-12 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrate was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-10 amount of sample captured on plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL-12 can be calculated.Kit Components:356618A: Microtiter Strips, 1x96 wells 356618B: Standard, 2x1vial356618C: Sample/Standard Dilution Buffer, 1x20ml356618D: Antibody (Biotin) (Concentrated), 1x120ul356618E: Antibody Dilution Buffer, 1x10ml356618F: Streptavidin (HRP) (SABC), 1x120ul356618G: SABC Dilution Buffer, 1x10ml356618H: TMB Substrate, 1x10ml356618J: Stop Solution, 1x10ml356618K: Wash Buffer, 25X, 1x30mlStorage and Stability:Store unopened *356618A at 4ºC. Once opened, store at -20ºC. Store *356618B powder at 4°C. Once reconstituted store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.Assay Summary:1. Wash plate 2 times before adding standards, samples and control (zero) to wells!2. Add 100ul standard or sample to each well for 90 minutes at 37°C3. Add 100ul Biotin antibody working solution to each well for 60 minutes at 37°C4. Aspirate and wash 3 times5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37°C6. Aspirate and wash 5 times7. Add 90ul TMB substrate. Incubate 10-20 minutes at 37°C8. Add 50ul Stop Solution. Read at 450nm immediately.9. Calculation of results
- 保存建议
- 建议收到IL-12 (Interleukin 12) BioAssay ELISA Kit (Human)产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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