The Toll-like receptor （TLR） family of proteins are characterized by a highly conserved Toll homology （TH） domain，which is essential for Toll-induced signal transduction. TLR1，as well as the other TLR family members，are type I transmembrane receptors that characteristically contain an extracellular domain consisting of several leucine-rich regions along with a single cytoplasmic Toll/IL-1R-like domain. TLR2 and TLR4 are activated in response to lipopolysacchride （LPS） stimulation，which results in the activation and translocation of NFkB and suggests that these receptors are involved in mediating inflammatory responses. TLR6 is highly homologous to TLR1，and like other members of TLR family，it induces NFkB signaling upon activation. Expression of TLR receptors is highest in peripheral blood leukocytes，macrophages，and monocytes.
应用类型
Western blotting （0.5-4 ug/ml）. However， the optimal conditions should be determined individually. Other applications have not been determined. The antibody recognizes ~97 kDa TLR5 in sample from human， mouse and rat origins. Reactivity to other species has not been tested. Blocking peptide （Cat.# 3555RBP-50） is available separately.
免疫原
Synthetic peptide surrounding amino acid 829 of human TLR5
来源宿主
兔
反应性
经检测验证，可与人、小鼠、大鼠种属来源的TLR5蛋白发生免疫反应.
保存建议
提供的TLR5抗体产品为100 ug 无色溶液，其浓度为0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA和0.01%硫柳汞的PBS缓冲液中.请将该TLR5抗体产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
