The Toll-like receptor （TLR） family in mammalian comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Up-to-date，ten TLRs （TLR1-10） have been described. TLR2 is differentially expressed in human cells. CD14+ monocytes expressed the highest level of TLR2 followed by CD15+ granulocytes，and CD19+ B-cells. CD3+ T-cells and CD56+ NK cells did not express TLR2. The expression of TLR2 on different cell types is regulated by different immune response modifiers. For example，LPS，GM-CSF，IL-1，and IL-10 up regulate TLR2，whereas IL-4，IFN-gamma，and TNF down regulate TLR2 expression in monocytes.
应用类型
Western blotting （0.5-4 ug/ml）. However， the optimal conditions should be determined individually. The antibody recognizes ~86 kDa TLR2 in Jurkat cell lysate. Reactivity to other species has not been tested. Blocking peptide （Cat.# 3552RBP-50） is available separately.
免疫原
Synthetic peptide surrounding amino acid 753 of mouse TLR2
来源宿主
兔
反应性
经检测验证，可与人、小鼠、大鼠、猴种属来源的TLR2蛋白发生免疫反应.
保存建议
提供的TLR2抗体产品为100 ug 无色溶液，其浓度为0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA和0.01%硫柳汞的PBS （pH 7.2）缓冲液中.请将该TLR2抗体产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
