Tau，a microtubule-binding protein which serves to stabilize microtubules in growing axons，is found to be hyperphosphorylated in paired helical filaments （PHF），the major fibrous component of neurofibrillary lesions associated with Alzheimer’s disease. Hyperphosphorylation of Tau is thought to be the critical event leading to the assembly of PHF. Six Tau protein isoforms have been identified，all of which are phosphorylated by GSK3. This presents the possibility that miscues in GSK3 signaling contribute to the onset of Alzheimer’s disease.
应用类型
Western blotting （0.5-4 ug/ml）. However， the optimal conditions should be determined individually. Other applications have not been determined. The antibody recognizes mainly the truncated Tau （~30 kDa） in sample from human， mouse and rat origins. Other isoforms of Tau at 35 kDa， 60 kDa and 80 kDa can also be recognized. Reactivity to other species has not been tested.
免疫原
Synthetic peptide surrounding amino acid 395 of rat Tau
来源宿主
兔
反应性
经检测验证，可与人、小鼠、大鼠、鸡、牛种属来源的Tau蛋白发生免疫反应.
保存建议
提供的Tau抗体产品为100 ug 无色溶液，其浓度为0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA和0.01%硫柳汞的PBS缓冲液中.请将该Tau抗体产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
