The matrix metalloproteinases （MMP） are a family of peptidase enzymes responsible for the degradation of extracellular matrix components，including collagen，gelatin，fibronectin，laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester，lipopolysaccharide （LPS） or staphylococcal enterotoxin B （SEB）. MMP catalysis requires both calcium and zinc. MMP-13 （also designated collagenase-3） is produced by breast carcinomas and degrades collagen type I，II，III.
应用类型
Western blotting （0.5-4 ug/ml）， immunoprecipitation and Immunohistochemistry （works with paraffin imbedded sections）. However， the optimal concentrations should be determined individually. The antibody recognizes the proenzyme （~60 kDa） and the cleaved fragments （34 kDa and 48 kDa） of MMP-13. Reactivity to other species has not been tested.
免疫原
Synthetic peptide surrounding amino acid 454 of rat MMP-13
来源宿主
兔
反应性
经检测验证，可与人、小鼠、大鼠、马、牛种属来源的MMP-13蛋白 发生免疫反应.
保存建议
提供的MMP-13抗体 产品为100 ug 无色溶液，其浓度为0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA和0.01%硫柳汞的PBS缓冲液中.请将该MMP-13抗体 产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
