The matrix metalloproteinases （MMP） are a family of peptidase enzymes responsible for the degradation of extracellular matrix components，including collagen，gelatin，fibronectin，laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester，lipopolysaccharide （LPS） or staphylococcal enterotoxin B （SEB）. MMP catalysis requires both calcium and zinc. MMP-12 （also designated macrophage metalloelastase） is produced in alveolar macrophages and degrades elastin. MMP-12 may contribute to elastin degradation occurring in granulomatous skin diseases and may also participate in macrophage migration through the epidermal and vascular basement membranes in inflammatory disorders.
应用类型
Western blotting （1-4 ug/ml）. However， the optimal concentrations should be determined individually.The antibody recognizes a ~50 kDa band from samples of human origin. Reactivity to other species has not been tested. Jurkat cell lysate （Cat.# 2401-100） can be used as a positive control.
免疫原
Synthetic peptide surrounding amino acid 460 of human MMP-12
来源宿主
兔
反应性
经检测验证，可与人、小鼠种属来源的MMP-12蛋白 发生免疫反应.
保存建议
提供的MMP-12抗体 产品为100 ug 无色溶液，其浓度为0.2 mg/ml，溶解于包含有50%甘油， 1% BSA和0.02%硫柳汞的PBS缓冲液中.请将该MMP-12抗体 产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
