- 中文名称
Transforming Growth Factor Beta 3 (TGF-beta 3), BioAssay ELISA Kit (Rat)
- 英文名字
- Transforming Growth Factor Beta 3 (TGF-beta 3), BioAssay ELISA Kit (Rat)
- 供应商
- USBiological
- 产品货号
- 353097
- 产品报价
- ￥1/96 Tests
- 产品说明书
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Transforming growth factor beta 3(TGF-beta 3) is a type of protein, known as a cytokine, which is involved in cell differentiation, embryogenesis and development. It belongs to a large family of cytokines called the Transforming growth factor beta superfamily. TGF-beta 3 is believed to regulate molecules involved in cellular adhesion and extracellular matrix(ECM) formation during the process of palate development. Without TGF-beta 3, mammals develop a deformity known as acleft palate. This is caused by failure of epithelial cells in both sides of the developing palate to fuse. TGF-beta 3 also plays an essential role in controlling the development of lungs in mammals, by also regulating cell adhesion and ECM formation in this tissue, and controls wound healing by regulating the movements of epidermal and dermal cells in injured skin. TGF-beta 3 activated Lef1 in the absence of beta-catenin(CTNNB1) via nuclear phospho-Smad2 and Smad4.Sample Type:Cell culture supernates, serum, plasma (EDTA) and urineIntended Use:For quantitative detection of activated rat TGF-beta 3 in cell culture supernates, serum, plasma (EDTA) and urine.Sensitivity:<10pg/mlRange:31.2-2000pg/mlSpecificity:Natural and recombinant rat TGF-beta 3Test Principle:This rat TGF-beta 3 BioAssay ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TGF-beta 3 has been precoated onto 96-well plates. Standards(sf21) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TGF-beta 3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat TGF-beta 3 amount of sample captured in plate.Kit Components:96-well plate precoated with rat TGF-beta 3 antibody, 1Lyophilized recombinant rat TGF-beta 3 standard, 10ng/tube×2Biotinylated rat TGF-beta 3 antibody, 130ul (dilution 1:100)Avidin-Biotin-Peroxidase Complex (ABC), 130ul (dilution 1:100)Sample diluent buffer, 30mlAntibody diluent buffer, 12mlABC diluent buffer, 12mlTMB color developing agent, 10mlTMB stop solution, 10mlStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对Transforming Growth Factor Beta 3 (TGF-beta 3), BioAssay ELISA Kit (Rat)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Transforming Growth Factor Beta 3 (TGF-beta 3), BioAssay ELISA Kit (Rat)产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
-
该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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