- 中文名称
Glycated Hemoglobin (HbA1c) BioAssay ELISA Kit (Human)
- 英文名字
- Glycated Hemoglobin (HbA1c) BioAssay ELISA Kit (Human)
- 供应商
- USBiological
- 产品货号
- 351956
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- The Glycated Hemoglobin (HbA1C) BioAssay ELISA Kit (Human) is a competitive inhibition immunoassay for the in vitro quantitative measurement of HbA1c in human plasma, serum and erythrocyte lysates.Detection Range:12.35-1,000ug/mlSensitivity:<4.95ug/mlPrecision:Intra-Assay CV: <10%Inter-Assay CV: <12%Test Principle:This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific for HbA1c has been pre-coated onto a microplate. A competitive inhibition reaction occurs between biotin-labeled HbA1c and unlabeled HbA1c (standards or samples) for limited binding sites on the pre-coated antibody. After incubation, the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is inversely proportional to the concentration of HbA1c in the sample. After addition of the substrate solution, the intensity of color developed is inversely proportional to the concentration of HbA1c in the sample.Kit Components:*351956A: Microtiter Plate, 96 wells, Pre-coated, ready to use.*351956B: Standard, 2x1vial 351956C: Standard Diluent, 1x20ml*351956D: Detection Reagent A, 1x120ul*351956E: Detection Reagent B, 1x120ul 351956F: Assay Diluent A, 1x12ml351956G: Assay Diluent B, 1x12ml351956H: TMB Substrate, 1x9ml351956K: Stop Solution, 1x6ml351956L: Wash Buffer, 30X, 1x20mlStorage and Stability:Store *351956A, *351956B, *351956D and *351956E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.Materials Required But Not Supplied:1. Microplate reader with 450 ± 10nm filter.2. Precision single or multi-channel pipettes and disposable tips.3. Eppendorf Tubes for diluting samples.4. Deionized or distilled water.5. Absorbent paper for blotting the microtiter plate.6. Container for Wash SolutionSample Preparation and Storage:Serum:Use a serum separator tube and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 20 minutes at approximately 1000xg. Assay freshly prepared serum immediately or store samples in aliquots at -20°C or -80°C for later use. Avoid repeated freeze/thaw cycles.Plasma:Collect plasma using EDTA as anticoagulant. Centrifuge samples for 15 minutes at 1000xg at 2-8°C within 30 minutes of collection. Remove plasma and assay immediately or store samples in aliquots at -20°C or -80°C for later use. Avoid repeated freeze/thaw cycles.Note: Serum/plasma samples require about 10-fold dilution (e.g., 20ul sample + 180ul PBS). Samples should be diluted using 0.01M PBS, pH 7.0-7.2.Erythrocyte Lysates:1. Centrifuge whole blood for approximately 20 minutes at 1000xg. Remove the supernatant and collect the cells.2. Wash cells three times in cold PBS (0.02M, pH 7.0-7.2).3. Resuspend the cells in ice-cold PBS. Perform a freeze-thaw cycle by freezing the cells at -20°C or lower and then thawing the cells with gentle mixing. Perform the freeze-thaw cycle 3 times.4. Centrifuge for 10 minutes at 5000xg at 2-8°C to remove cellular debris.5. Assay the supernatant immediately or store samples in aliquots at -20°C or -80°C for later use. Avoid repeated freeze/thaw cycles.Assay Procedure Summary:1. Prepare all reagents, samples and standards.2. Add 50ul standard or sample to each well, then add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C.3. Aspirate and wash 3 times.4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.5. Aspirate and wash 5 times.6. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37°C.7. Add 50ul Stop Solution. Read absorbance at 450nm immediately.
- 产品特点
- 针对Glycated Hemoglobin (HbA1c) BioAssay ELISA Kit (Human)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Glycated Hemoglobin (HbA1c) BioAssay ELISA Kit (Human)产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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