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中文名称

Soluble Tumor Necrosis Factor-like Weak Inducer of Apoptosis (sTWEAK) BioAssay ELISA Kit (Human)

英文名字
Soluble Tumor Necrosis Factor-like Weak Inducer of Apoptosis (sTWEAK) BioAssay ELISA Kit (Human)
供应商
USBiological
产品货号
351697
产品报价
¥1/48Tests   ¥1/96Tests   ¥1/24Tests
产品说明书
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产品新闻
背景资料
USBiological品牌是全球有名的抗原抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理,是行业中少有的致力于服务客户,帮助客户,且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应,专业的产品和解决方案支持,稳健快捷的交货周期,优质放心的售后服务。我们致力于为您提供有价值的产品和服务,在意您的成功!
产品描述
This protein is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein is a ligand for the FN14/TWEAKR receptor. This cytokine has overlapping signaling functions with TNF, but displays a much wider tissue distribution. This cytokine can induce apoptosis via multiple pathways of cell death in a cell type-specific manner. This cytokine is also found to promote proliferation and migration of endothelial cells, and thus acts as a regulator of angiogenesis. Some transcripts that skip the last exon of this gene and continue with the second exon of the neighboring TNFSF13 gene have been identified; such read-through transcripts are contained in GeneID 407977, TNFSF12-TNFSF13.Sample Type:Serum, plasma, tissue homogenatesIntended Use:For the quantitative determination of human soluble tumor necrosis factor-like weak inducer of apoptosis (sTWEAK) concentrations in serum, plasma, tissue homogenates.Sensitivity:7.8pg/mlRange:31.25-2000pg/mlSpecificity:This assay has high sensitivity and excellent specificity for detection of human sTWEAK. No significant cross-reactivity or interference between human sTWEAK and analogues was observed.Test Principle:This BioAssay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for sTWEAK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any sTWEAK present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for sTWEAK is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of sTWEAK bound in the initial step. The color development is stopped and the intensity of the color is measured.Kit Components:Assay plate (12x8 coated microwells), 1x96 wellsStandard (Freeze dried), 2x vialsBiotin-antibody (100X), 1x120ulHRP-avidin (100X), 1x120ulBiotin-antibody Diluent, 1x15mlHRP-avidin Diluent, 1x15mlSample Diluent, 1x50mlWash Buffer (25X), 1x20mlTMB Substrate, 1x10mlStop Solution, 1x10mlAdhesive Strip (For 96 wells), 4x stripsInstruction manualStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
产品特点
针对Soluble Tumor Necrosis Factor-like Weak Inducer of Apoptosis (sTWEAK) BioAssay ELISA Kit (Human)该产品的特点优势,欢迎查阅官网提供的产品说明书。
保存建议
建议收到Soluble Tumor Necrosis Factor-like Weak Inducer of Apoptosis (sTWEAK) BioAssay ELISA Kit (Human)产品后将其置于4°C/-20°C保存。
其他
Usbiological公司是美国著名的抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品,为科研用户提供了诸多超值选择。
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