- 中文名称
Rotavirus Antigen (RV Ag) BioAssay Qualitative ELISA Kit (Bovine)
- 英文名字
- Rotavirus Antigen (RV Ag) BioAssay Qualitative ELISA Kit (Bovine)
- 供应商
- USBiological
- 产品货号
- 351689
- 产品报价
- ￥1/48Tests ￥1/96Tests ￥1/24Tests
- 产品说明书
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Intended Use:This Rotavirus Antigen (RV Ag) ELISA Kit is for the qualitative determination of RV Ag in bovine feces. This kit is intended for research use only and may not be used for therapeutic or diagnostic applicationsPrinciple of the Test:This assay employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to RV Ag. Samples are pipetted into the wells with Horseradish Peroxidase (HRP)-conjugated antibody. Any virus antigen present in the samples will bind to the pre-coated specific antibody. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RV Ag bound in the initial step. The color development is stopped and the intensity of the color is measured.Kit Components:351689A: Microtiter Strips, 1x96 wells. Pre-coated.351689B: Negative Control, 1x800ul351689C: Positive Control, 1x00ul351689D: HRP Conjugate, 1x126ml351689E: Wash Buffer, 30X, 1x6mll 351689F: Substrate A, 1x5ml 351689G: Substrate B, 1x5ml351689H: Stop Solution, 1x5ml Storage and Stability:Store kit at 4°C. The unopened kit is stable for 6 months after receipt. Once opened, the kit is stable for up to 2 weeks at 4°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.Assay Procedure:Bring all reagents and samples to room temperature before use. Centrifuge the samples again after thawing before the assay. It is recommended that all samples be assayed in duplicate.1. Prepare all reagents, and *samples as directed in the previous sections. 2. Determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc; store unused wells at 4°C. 3. Set a Blank well (without any solution), three Negative Control wells and two Positive Control wells.4. Add 50ul of Negative Control, Positive Control or Sample per well, then add 50ul of HRP Conjugate to each well except the Blank well. Cover with the provide adhesive strip. Incubate for 15 minutes at room temperature.5. Aspirate each well. Add 50ul Wash Buffer to each well.6. Wash by filling each well with 250ul ddH2O using a squirt bottle, multi-channel pipette, manifold dispenser or autowasher, and let it stand for 30 seconds; complete removal of liquid at each step is essential to good performance. Repeat the process ten times for a toal of ten washes. After the last wash, remove any remaining liquid by aspirating or decanting. Invert the plate and blot it against clean paper towels.7. Add 50ul of Substrate A and 50ul Substrate B to each well. Incubate for 10 minutes at room temperature. Protect from light.8. Add 50ul of Stop Solution to each well; gently tap the plate to ensure thorough mixing.9. Determine the optical density of each well within 10 minutes using a microplate reader set to 450nm. Take blank well as zero.Interpretation of Results:The presence or absence of Rotavirus Antigen is determined by comparing the absorbance of the samples with those of the controls.A Cut-off value is defined as the average Negative Control OD value plus 0.1. If the average Negative Control OD valuel is <0.05, calculate it as 0.05.1. The Negative Control OD value should be less than 0.1 and the Positive Control OD value should be greater than 0.8. Otherwise, the test must be repeated.2. Determination of results:If ODsample is ≥ Cut-off Value: Test is PositiveIf ODsample is < Cut-off Value: Test is Negative If OD sample is close to the Cut-off Value, we recommend repeating the experiment.
- 产品特点
- 针对Rotavirus Antigen (RV Ag) BioAssay Qualitative ELISA Kit (Bovine)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Rotavirus Antigen (RV Ag) BioAssay Qualitative ELISA Kit (Bovine)产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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