- 中文名称
Plasminogen (PLG) BioAssay ELISA Kit (Mouse)
- 英文名字
- Plasminogen (PLG) BioAssay ELISA Kit (Mouse)
- 供应商
- USBiological
- 产品货号
- 351682
- 产品报价
- ¥1/48Tests ¥1/96Tests ¥1/24Tests

- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金,在线支付宝及网银支付,或直接与我们电话联系400-6800-868
- 产品新闻

- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理,是行业中少有的致力于服务客户,帮助客户,且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应,专业的产品和解决方案支持,稳健快捷的交货周期,优质放心的售后服务。我们致力于为您提供有价值的产品和服务,在意您的成功!
- 产品描述
- Plasminogen (PLG), BioAssay ELISA Kit (Mouse) is a competitive ELISA intended for the quantitative determination of PLG concentration in mouse serum, plasma and tissue homogenates.Sensitivity:<15.6ng/mlRange:62.5-4000ng/mlPrecision:Intra-assay CV: <8%Inter-assay CV: <10%Principle of the Assay:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with PLG. Standards or samples are added to the appropriate microtiter plate wells with HRP-conjugated anti-PLG antibody. A competitive inhibition reaction is launched between pre-coated PLG and PLG in the standards or samples. A substrate solution is added to the wells; the color development is stopped and the intensity of the color is measured. The intensity of the color is inversely proportional to the amount of PLG in the samples.Kit Components:227665A: Microtiter Strips (12 x 8 microwells). Pre-coated with PLG; ready to use. 227665B: Standard (Lyophilized), 2x1 vials227665C: HRP Conjugate (100X Concentrate), 1x60ul227665D: HRP Conjugate Diluent, 1x10ml227665E: Sample Diluent 2x20ml227665F: Wash Buffer (25X Concentrate), 1x20ml227665G: TMB Substrate, 1x10ml227665H: Stop Solution, 1x10ml227665J: Wash Buffer (25X Concentrate), 1x20mlStorage and Stability:Unopened kit: Store at 4°C. Unused kit is stable for 6 months after receipt. Opened Kit: Once opened, the following kit components are stable for up to 1 month at 4°C providing this is within the expiration date of the kit: HRP conjugate diluent, Sample diluent, Wash buffer, TMB substrate and Stop solution. The coated plate, standard and HRP conjugate may be stored at 4°C for up to one month; store the standard and HRP conjugate at -20°C if the kit will not be used within one month. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.Assay Summary:1. Prepare reagents, working standards and samples as instructed.2. Set a Blank well without any solution3. Add 50ul Standard or sample to each well.4. Add 50ul HRP Conjugate to each well (do not add any to the blank well).5. Incubate for 60 minutes at 37°C.6. Aspirate and wash 5 times.7. Add 90ul TMB substrate to each well8. Incubate for 20 minutes at 37°C. Protect from light12. Add 50ul Stop Solution to each well. Read at 450nm within 5 minutes.
- 产品特点
- 针对Plasminogen (PLG) BioAssay ELISA Kit (Mouse)该产品的特点优势,欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Plasminogen (PLG) BioAssay ELISA Kit (Mouse)产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品,为科研用户提供了诸多超值选择。
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- 注意
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