- 中文名称
Lipoprotein-associated Phospholipase A2 (Lp-PLA2) BioAssay ELISA Kit (Human)
- 英文名字
- Lipoprotein-associated Phospholipase A2 (Lp-PLA2) BioAssay ELISA Kit (Human)
- 供应商
- USBiological
- 产品货号
- 351661
- 产品报价
- ￥1/48Tests ￥1/96Tests ￥1/24Tests
- 产品说明书
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Sample Type:Serum, plasma, tissue homogenatesIntended Use:For the quantitative determination of human lipoprotein-associated phospholipase A2 (Lp-PLA2) concentrations in serum, plasma, tissue homogenates.Sensitivity:0.39ng/mlRange:1.56-100ng/mlSpecificity:This assay has high sensitivity and excellent specificity for detection of human Lp-PLA2. No significant cross-reactivity or interference between human Lp-PLA2 and analogues was observed.Principle of the Assay:This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Lp-PLA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Lp-PLA2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Lp-PLA2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Lp-PLA2 bound in the initial step. The color development is stopped and the intensity of the color is measured. Kit Components:351661A: Microtiter Strips, 12x8 Microwell Strips. Pre-coated; ready to use. 351661B: Standard (lyophilized), 2 vials351661C: Biotin-Antibody (100x Concentrate), 1x120ul351661D: HRP-Avidin, (100x Concentrate), 1x120ul351661E: Biotin-Antibody Diluent, 1x15ml351661F: HRP-Avidin Diluent, 1x15ml351661G: Sample Diluent, 1x50ml351661H: Wash Buffer (25X Concentrate), 1x20ml351661J: TMB Substrate, 1x10ml351661K: Stop Solution, 1x10mlStorage and Stability:Store all kit components at 4°C. Unopened kit is stable for 6 months after receipt. Once opened, the kit is stable for 1 month provided this is within the expiration date of the kit.Opened Kit: Coated plate may be stored at 4°C for up to 1 month, sealed in the aluminum foil bag. Standard, Biotin-Antibody and HRP-Avidin may be stored at 4°C or -20°C for up to 1 month. The rest of the components may be stored at 4°C for up to 1 month.For maximum recovery of contents, centrifuge the original vials after thawing and prior to removing the cap.Assay Summary:1. Prepare reagents, standards and samples as instructed.2. Add 100ul standard or sample to each well. Incubate for 2 hours at 37°C3. Remove the liquid of each wee; do not wash.4. Add 100ul Biotin-Antibody (1x) to each well. Incubate for 1 hour at 37°C5. Aspirate and wash 3 times.6. Add 100ul HRP-Avidin (1x) to each well. Incubate for 1 hour at 37°C7. Aspirate and wash 5 times.8. Add 90ul TMB substrate to each well Incubate for 15-30 minutes at 37°C. Protect from light.9. Add 50ul Stop Solution to each well. Read at 450nm within 5 minutes.
- 产品特点
- 针对Lipoprotein-associated Phospholipase A2 (Lp-PLA2) BioAssay ELISA Kit (Human)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Lipoprotein-associated Phospholipase A2 (Lp-PLA2) BioAssay ELISA Kit (Human)产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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