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细胞分析试剂盒
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中文名称

IFN-gamma (Interferon-gamma), BioAssay ELISA Kit (Mouse)

英文名字
IFN-gamma (Interferon-gamma), BioAssay ELISA Kit (Mouse)
供应商
USBiological
产品货号
350996
产品报价
¥1/96Tests
产品说明书
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产品新闻
背景资料
USBiological品牌是全球有名的抗原抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理,是行业中少有的致力于服务客户,帮助客户,且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应,专业的产品和解决方案支持,稳健快捷的交货周期,优质放心的售后服务。我们致力于为您提供有价值的产品和服务,在意您的成功!
产品描述
Interferon ­gamma (IFN­gamma) exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine. Mature mouse IFNgamma exists as a noncovalently linked homodimer of 20­25kD variably glycosylated subunits. It shares 86% amino acid sequence identity with rat IFN­gamma and 38%-­44% with human, porcine, and rhesus IFN­gamma. IFNgamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, up­regulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects.Sample Type:Serum and plasmaIntended Use:The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of IFN-gamma in mouse serum, plasma; and other biological fluids not being tested.Sensitivity:4pg/mlRange:16.4-4000pg/mlSpecificity:This assay has high sensitivity and excellent specificity for detection of mouse IFN-gamma.Precision:Intra CV<10%; Inter CV <15%Test Principle:This assay employs the fast quantitative sandwich enzyme immunoassay technique. Antibody specific for mouse IFN-gamma has been pre-coated onto a microplate. Standards, samples and mouse IFN-gamma mix are pipetted into the wells and any IFN-gamma present is bound by the immobilized antibody. Following a wash to remove any unbound reagent, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IFN-gamma bound in the initial step. The color development is stopped and the intensity of the color is measured.Kit Components:Assay plate (12x8 coated Microwells)-1Standard-2x10uLMouse IFN-gamma Mix-4x8uLAssay diluent (5x concentrate)-1x15mLWash buffer (20x concentrate)-1x25mLTMB substrate-1x12mLStop solution-1x8mLAdhensive strip-2Instruction manual-1.Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
产品特点
针对IFN-gamma (Interferon-gamma), BioAssay ELISA Kit (Mouse)该产品的特点优势,欢迎查阅官网提供的产品说明书。
保存建议
建议收到IFN-gamma (Interferon-gamma), BioAssay ELISA Kit (Mouse)产品后将其置于4°C/-20°C保存。
其他
Usbiological公司是美国著名的抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品,为科研用户提供了诸多超值选择。
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