TrkA，the high affinity Nerve Growth Factor （NGF） receptor，autophosphorylates on tyrosine to activate multiple effectors. Phosphorylation at Tyr490 is required for Shc association and activation of the Ras-MAP kinase cascade. Residues Tyr674/675 lie within the catalytic domain，and phosphorylation at these sites reflects Trk kinase activity.
应用类型
Western blotting （1 ug/ml）， immunoprecipitation （4-8 ug/ml）， and immunocytochemistry （10-20 ug/ml）. However， the optimal conditions should be determined individually. The antibody detects 140 kDa TrkA only when phosphorylated at Tyr490.
免疫原
Synthetic phospho-peptide corresponding to resides surrounding Tyr490 of human TrkA
