PKC （Protein kinase C） is a calcium-dependent and phospholipid-dependent enzyme that is activated in vivo by the lipid diacylglycerol. PKC has been shown to be involved in signal transduction and various aspects of neoplastic transformation. PKC contains an amino terminal regulatory domain and a carboxy terminal catalytic domain joined by a hinge region. Cleavage of protein kinase C in vitro results in the generation of two functional fragments corresponding to these two domains.
应用类型
The antibody can be used in Western blotting （1-2 ug/ml）. However， the optimal concentrations should be determined individually. The antibody recognizes several Phosphorylated PKC （Ser660） isoforms of human， mouse， and rat origins. Reactivity to other species has not been tested.
免疫原
Synthetic phospho-peptide surrounding amino acid Ser660 of human PKC beta II
来源宿主
兔
反应性
经检测验证，可与人、小鼠、大鼠种属来源的Phospho-PKC蛋白 发生免疫反应.
保存建议
提供的Phospho-PKC抗体 产品为100 ug 无色溶液，其浓度为0.5 mg/ml，溶解于包含有50%甘油， 1% BSA和0.02% 叠氮钠的PBS缓冲液中.请将该Phospho-PKC抗体 产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
