Five ionomycin-inducible complementary cDNAs，designated PAR1，2，3，4 and 5，have been isolated from the prostate cancer cell line AT-3. Nucleotide sequencing identified PAR1 as the rat homolog of monkeyP-1，PAR2 as the injury-inducible gene HB-EGF，and PAR3 as the serum-induced gene cyr-61. PAR4 and PAR5 sequences were not found to correspond to any previously described proteins. PAR4 （prostate apoptosis response-4） is specifically expressed by cells entering apoptosis and is not inducible by growth factor stimulation，oxidative stress and necrosis，or growth arrest. The PAR4 gene encodes a 38 kDa protein with a putative nuclear localization signal and carboxy terminal leucine zipper.
应用类型
Western blotting （0.5-4 ug/ml）. However， the optimal conditions should be determined individually. The antibody recognizes PAR4 in samples from of human， mouse， and rat origins. Reactivity to other species has not been tested.
免疫原
Synthetic peptide surrounding amino acid 327 of human Par4
来源宿主
兔
反应性
经检测验证，可与人、小鼠、大鼠、狗、马种属来源的PAR4蛋白发生免疫反应.
保存建议
提供的PAR4抗体产品为100 ug 无色溶液，其浓度为0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA和0.01%硫柳汞的PBS缓冲液中.请将该PAR4抗体产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
