GADD153 has been described as a growth arrest and DNA damage-inducible gene that encodes a C/EBP-related nuclear protein. Expression of GADD153 is induced by a variety of cellular stresses，including nutrient deprivation and metabolic perturbations. GADD153 functions to block cells in G1 to S phase in cell cycle progression and acts by dimerizing with other C/EBP proteins to direct GADD153 dimers away from “classical” C/EBP binding sites. Thus GADD153 acts as a negative modulator of C/EBP-like proteins in certain terminally differentiated cells.
应用类型
Western blotting （0.5-4 ug/ml）， immunoprecipitation （10-20 ug/ml）， and Immunohistochemistry （10-20 ug/ml）. However， the optimal concentrations should be determined individually. The antibody recognizes 25 kDa GADD153 of human， mouse， and rat origins.
免疫原
Synthetic peptide surrounding amino acid 124 of human GADD153
来源宿主
兔
反应性
经检测验证，可与人、小鼠、大鼠种属来源的GADD153蛋白发生免疫反应.
保存建议
提供的GADD153抗体产品为100 ug 无色溶液，其浓度为0.2 mg/ml，溶解于包含有30%甘油， 0.5% BSA和0.01%硫柳汞的PBS缓冲液中.请将该GADD153抗体产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
