Caspase family of cysteine proteases play a key role in apoptosis. Caspase-10 is expressed in many tissues and cell lines，and has been identified as caspase-10/a （short form） and caspase-10/b （long form），respectively. Similar as other caspases，caspase-10 is also synthesized as an inactive pro-enzyme that is processed in cells undergoing apoptosis. Activated caspase-10 activates caspase-3，-4，-6，-7，-8，and –9，which are then involved in proteolytic cleavage of many cellular proteins. Caspase-10 has been shown involving in the Fas and TNFR1 induced apoptosis.
应用类型
Western blotting （0.5-4 ug/ml）. However， the optimal conditions should be determined individually. The antibody detects the proform （~55 kDa）， intermediates （30 kDa and 34 kDa）， and cleaved form （~20 kDa） of caspase-10 on SDS-PAGE immunoblots.
免疫原
Synthetic peptide surrounding amino acid 404 of human caspase-10
来源宿主
兔
反应性
经检测验证，可与人、小鼠、大鼠种属来源的Caspase-10蛋白发生免疫反应.
保存建议
提供的Caspase-10抗体产品为200 ug 无色溶液，其浓度为0.2 mg/ml，溶解于包含有30%甘油， 0.5% BSA， 0.01%硫柳汞的PBS （pH 7.2）缓冲液中.请将该Caspase-10抗体产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
