Calpains are cytosolic，calcium-dependent，cysteine proteases that are ubiquitously expressed in mammalian and avian tissues. Calpain-1 and 2 consist of a common small subunit and a large variable subunit. Domains in the large subunit include the amino terminal domain I，the proteinase domain II，domain III，and the EF-hand domain IV. Type I calpain requires micromolar amounts of calcium，while type II calpain requires millimolar amounts of calcium.
应用类型
Western blotting （1:200-1000 dilutions）. However， the optimal concentrations should be determined individually. The anti-calpain-2 antibody recognizes the 80 kDa Calpain-2. It does not cross-react with other calpains
免疫原
Synthetic peptide mapping to the C-terminus of the domain III of human Calpain-2 large subuni
来源宿主
兔
反应性
经检测验证，可与人、小鼠、大鼠种属来源的Calpain-2蛋白发生免疫反应.
保存建议
提供的Calpain-2抗体产品为100 ul 无色溶液，其浓度为N/A，溶解于包含有50%甘油， 1% BSA和0.02%硫柳汞的PBS缓冲液中.请将该Calpain-2抗体产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
