Bcl-xL is a 30 kDa anti-apoptotic protein belonging to the Bcl-2 family. Bcl-xL prevents apoptosis through two different mechanisms: by heterodimerization with an apoptotic protein to inhibit its apoptotic effect，and by its direct pore-forming effect on the outer-membrane of mitochondria to help maintain a normal membrane state under stress conditions. Bcl-xL can be cleaved by caspases upon apoptotic activation，which converts Bcl-xL from an antiapoptotic protein to a proapoptotic cleavage product. The cleavage product of Bcl-xL forms cytochrome c-releasing pores in the mitochondrial membrane to facilitate the apoptosis signaling pathway.
应用类型
Western blotting （0.5-4 ug/ml） and immunoprecipitation （20 u/ml）. However， the optimal conditions should be determined individually. The antibody detects the 30 kDa Bcl-xL protein. It does not react with other Bcl-2 family members.
免疫原
Synthetic peptide surrounding amino acid 56 of human Bcl-xL
来源宿主
兔
反应性
经检测验证，可与人、小鼠、大鼠种属来源的Bcl-xL蛋白发生免疫反应.
保存建议
提供的Bcl-xL抗体产品为100 ug 无色溶液，其浓度为0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA， 0.01%硫柳汞的PBS （pH 7.2）缓冲液中.请将该Bcl-xL抗体产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
