Receptor interacting protein including RIP and RIP2/RICK mediate apoptosis induced by TNFR1 and Fas，two prototype members in the death receptor family. A novel member in the RIP kinase family was recently identified and designated RIP3. RIP3 contains N-terminal kinase domain but，unlike RIP or RIP2，lacks the c-terminal death or CARD domain. RIP3 binds to RIP and TNFR1，and mediates TNF-R1 mediated apoptosis，and attenuates RIP and TNFR1 induced NFkB activation. Overexpression of RIP3 induces apoptosis and NFkB activation.
应用类型
Western blotting （0.5-4 ug/ml） and in Immunohistochemistry （10-20 ug/ml）. Rat kidney tissue section can be used as a positive control. An approximately 57 kDa band should be detected.
免疫原
Synthetic peptide corresponding to residues surrounding amino acid 476 of mouse RIP3
来源宿主
兔
反应性
经检测验证，可与小鼠、大鼠种属来源的RIP3蛋白发生免疫反应.
保存建议
提供的RIP3抗体产品为100 ug 无色溶液，其浓度为0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA， 0.01%硫柳汞的PBS （pH 7.2）缓冲液中.请将该RIP3抗体产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
