Gi2 is a 40-41 kDa alpha-subunit of heterotrimeric protein that shares sequence homology with Gi3 and Gi1. All these G proteins can be ADP-ribosylated at the carboxy terminal Cys of the CAAX sequence，interfering with receptor coupling. Gi2 is expressed widely in tissues and cell lines. Activation of PLC-B by Gi-coupled receptors is the result of release of GBg subunits from Gi. Gi2 regulates potassium channel activation. Mutation at Arg179 has been identified in tumors. This and the Q205L mutant inhibit adenylyl cyclase.
应用类型
Western blot, Immunoprecipitation
免疫原
Synthetic peptide surrounding amino acid 229 of human Gai2
