Nestin is a type IV intermediate filament protein expressed by neuroepithelial stem cells and which has been proposed to represent a marker for putative islet stem cells. It is a high molecular weight protein with a terminus greater than 500 residues. Nestins are expressed by several types of cells during development，mostly in dividing cells of the Central Nervous System，Peripheral Nervous System and myogenic tissues. It also represents the progenitor population of neural stem cell origin. The role of nestin in dynamic cells，particularly structural organization of the cell，appears strictly regulated by phosphorylation，especially its integration into heterogeneous intermediate filaments together with vimentin or -internexin. Nestin has recently received attention as a marker for detecting newly formed endothelial cells.
应用类型
Western blot analysis （3-5 ug/ml） and IHC-paraffin （5 ug/ml）.
免疫原
Amino acids corresponding to 1450-1500 of human Nestin
来源宿主
兔
反应性
经检测验证，可与人、黑猩猩种属来源的Nestin蛋白发生免疫反应
保存建议
提供的100 ug Nestin抗体产品为无色溶液其浓度为0.5 mg/ml溶解于包含有0.05% BSA和0.05%叠氮化钠的PBS缓冲液中.请将该Nestin抗体产品置于-20 °C冷冻保存，建议分装使用以避免反复冻融.
