Members of Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 （BH3） domain is a potent death domain. BH3 domain containing pro-apoptotic proteins，including Bad，Bax，Bid，Bik，Hrk，Nip3，and Bim，form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bnip3L，Bnip3，and Nix （for Nip3-like protein X）. Bnip3L/Bnip3/Nix is a homolog of the E1B 19K/Bcl-2 binding and pro-apoptotic protein Bnip3. Overexpression of Bnip3L induces apoptosis. Bnip3L interacts with and overcomes supression by Bcl-2 and Bcl-xL. Bnip3L is localized in mitochondria. The message RNA of Bnip3L and Bnip3 form a new subfamily of the proapoptotic mitochondrial proteins.
应用类型
The antibody can be used for Western blot analysis （0.5-2 ug/ml） and immunohistochemistry （2-10 ug/ml）. The optimal conditions should be determined individually. Other applications have not been tested.
免疫原
Synthetic peptide surrounding amino acid 80 of human Bnip3L.
