Caspases are synthesized as inactive pro-enzymes that are processed to active form in cells undergoing apoptosis. Caspase-9 is an important member of the caspase family. Upon induction of apoptosis，Cytochrome c released from mitochondria associates with pro-caspase-9 （47 kDa） and Apaf-1. The complex processes pro-caspase-9 into a large subunit （37 kDa/17 kDa） and a small subunit （10 kDa）. Cleaved caspase-9 further processes other caspases including caspase-3 and caspase-6，to initiate a caspase cascade leading to apoptosis. The affinity purified antibody recognizing the active forms of caspase-9 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells.
应用类型
Western blotting （0.5-4 ug/ml）， Immunocytochemistry （10-20 ug/ml）， and Immunohistochemistry （10-20 ug/ml）. However， the optimal concentrations should be determined individually. The anti-active caspase-9 antibody recognizes the cleaved caspase-9 （37 kDa/17 kDa）. It does not recognize full-length caspase-9 or any other caspases.
免疫原
Synthetic peptide corresponding to amino-terminal residues adjacent to Asp353 of rat caspase-9
