Caspases are synthesized as inactive pro-enzymes that are processed to active form in cells undergoing apoptosis. Caspase-9 is an important member of the caspase family. Upon induction of apoptosis，Cytochrome c released from mitochondria associates with pro-caspase-9 （47 kDa） and Apaf-1. The complex processes pro-caspase-9 into a large subunit （37 kDa/17 kDa） and a small subunit （10 kDa）. Cleaved caspase-9 further processes other caspases including caspase-3 and caspase-6，to initiate a caspase cascade leading to apoptosis. The affinity purified antibody recognizing the active forms of caspase-9 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells.
应用类型
Western blotting （0.5-4 ug/ml） and immunoprecipitation （10-20 ug/ml）. However， the optimal concentrations should be determined individually. The anti-active caspase-9 antibody recognizes only the cleaved caspase-9 （37 kDa）. It does not recognize full-length caspase-9 or any other caspases.
免疫原
Synthetic peptide mapping to the N-terminus adjacent to Asp330 of human caspase-9
来源宿主
兔
反应性
经检测验证，可与人种属来源的Caspase-9 （Active）蛋白发生免疫反应.
保存建议
提供的Caspase-9 （Active）抗体产品为100 ug 无色溶液，其浓度为0.2 mg/ml，溶解于包含有50%甘油， 0.5% BSA和0.02%硫柳汞的PBS缓冲液中.请将该Caspase-9 （Active）抗体产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
