Caspase-7 has been identified as a major contributor to the execution of apoptosis. Caspase-7 is cleaved by many enzymes，including caspase-3，-6，-8，-9 and granzyme B. Active caspase-7 consists of a heterotetramer of two large and two small subunits. As an effector caspase，caspase-7 cleaves many of the same substrates as caspase-3，including poly （ADP-ribose） polymerase （PARP）. The active caspase-7 antibody provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells.
应用类型
Western blotting （0.5-4 ug/ml）， immunoprecipitation （10-20 ug/ml）， and Immunohistochemistry （10-20 ug/ml）. However， the optimal concentrations should be determined individually. The antibody detects only the large subunit of cleaved caspase-7. The antibody does not recognize full length of caspase-7 or other caspases.
免疫原
Synthetic peptide surrounding amino acid 195 of human caspase-7
