Lamins are classified into A- and B- type isoforms. Specifically，four distinct lamin isoforms have been identified: A，B1，B2，and C. Lamin A and C are alternatively spliced products of a single gene，whereas lamin B1 and B2 are encoded by distinct genes. During mitosis，lamins are phosphorylated by p34cdc2 and solubilized prior to chromosome condensation and nuclear breakdown. A direct role for lamins in chromosome condensation is suggested by the fact that lamin B binds to specific DNA sequence motifs termed matrix attachment regions （MARs）. Mars mediate the interaction of chromatin with the nuclear matrix. Importantly，some of the same processes that involve mitotic chromosome condensation and nuclear envelop breakdown are also activated during apoptosis. It appears that chromosome condensation during apoptosis is accompanied by degradation of lamin protein. Lamin degradation occurs prior to DNA fragmentation and this degradation is useful as an apoptotic marker.
应用类型
Western blotting （0.5-2 ug/ml）， immunoprecipitation （5 ug/ml）， immuno-fluorescence （10-15 ug/ml）， and ELISA （0.2-1 ug/ml）. However， the optimal conditions should be determined individually.
免疫原
Recombinant human Lamin B
来源宿主
小鼠
反应性
经检测验证，可与人、小鼠、大鼠种属来源的Lamin B1蛋白发生免疫反应.
保存建议
提供的Lamin B1抗体产品为100 ug 无色溶液，其浓度为0.2 mg/ml，溶解于包含有1 mg/ml BSA和1.5 mM 叠氮钠和50%甘油.的PBS缓冲液中.请将该Lamin B1抗体产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
