- 中文名称
Egg White Residue, BioAssay ELISA Kit (Gallus gallus)
- 英文名字
- Egg White Residue, BioAssay ELISA Kit (Gallus gallus)
- 供应商
- USBiological
- 产品货号
- 302884
- 产品报价
- ￥1/1Kit
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Hen egg (Gallus gallus) is very rich of proteins and represents an important food source for humans. While proteins of egg yolk only have minor allergenicity, many proteins of egg white are known to be allergenic. In addition to ovalbumin, ovotransferrin, lysozyme and livetin, ovomucoid represents the most important allergen. Unlike the other allergens ovomucoid is heat stable and can resist common production processes like baking. For allergic persons the consumption of egg white represents a critical problem. Already very low amounts of the allergen can cause allergic reactions, which may lead to anaphylactic shock in severe cases. Because of this, egg allergic persons must strictly avoid the consumption of eggs or egg containing food. Non-declared addition of egg in food is hazardous for allergic people. Cross contamination, mostly in consequence of the production process is often noticed. The chocolate production process is a representative example. For the detection of egg white protein residues, sensitive detection systems are required. Egg is one of the more frequent causes of food hypersensitivity in infants and young children. Studies have shown that ovomucoid also known as trypsin inhibitor from chicken egg white is a major allergen of egg whites. Intended Use:The United States Biological’s Egg White ELISA BioAssay Kit represents a highly sensitive detection system for egg white protein based on ovomucoid and is particularly capable of the quantification of egg white residues in pasta, bakery products, sausage and chocolate. Test Principle:This test is based on the principle of enzyme linked immunosorbent assay. An antibody directed against ovomucoid (egg white protein) is bound on the surface of a microtiter plate. Ovomucoid-containing samples or standards are given into the wells of the microtiter plate. After 20 minutes incubation at room temperature, the wells are washed with diluted washing solution to remove unbound material. A peroxidase-conjugated antibody directed against ovomucoid is given into the wells and after 20 minutes of incubation the plate is washed again. A substrate solution is added and incubated for 20 minutes, resulting in the development of a blue color. The color development is inhibited by the addition of a stop solution, and the color turns yellow. The yellow color is measured spectrophotometrically at 450nm. The concentration of ovomucoid (egg white protein) is directly proportional to the color intensity of the test sample.Sensitivity:The limit of detection (LOD): 0.05ppm. The limit of quantification (LOQ): 0.4ppm. Precision:Intra-assay Precision: CV: 4-9%Inter-assay Precision: CV: 3-7%Inter-lot Precision: CV: 5-11% Kit Components:302884A: Microtiter plate, 12 x 8 strip wells coated with anti-ovomucoid antibody302884B: Standard, 0ppm, 1x1ml; dyed red and ready to use302884C: Standard, 1ppm, 1x1ml; dyed red and ready to use302884D: Standard, 4ppm, 1x1ml; dyed red and ready to use302884E: Standard, 10ppm, 1x1ml; dyed red and ready to use302884F: Standard, 40ppm, 1x1ml; dyed red and ready to use302884G: Conjugate (anti-ovomucoid IgG-HRP), 1x15ml; dyed red and ready to use302884H: Substrate Solution (TMB), 1x15ml; ready to use302884J: Stop Solution (0.5M H2SO4), 1x15ml, ready to use302884K: Extraction and Sample Dilution Buffer, 10X (Tris concentrate), 2x120ml; dyed red. If during the cold storage crystals precipitate, the concentrate should be warmed up to 37°C for 15 minutes. Dilute 1+9 with distilled water. Stored at 4°C the diluted buffer is stable for at least one week. If during the cold storage crystals precipitate, the concentrate should be warmed up to 37°C for 15 minutes.302884L: Wash Solution, 10X (PBS + Tween 20), 1x60ml. Dilute 1+9 with distilled water. Stored at 4°C, the diluted buffer is stable for at least 4 weeks. Storage and Stability:Store components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.Sample Preparation:Due to high risk of cross-contamination, all applied equipment like applicator, mortar, glass vials etc. have to be cleaned thoroughly before and after each sample. To identify possible cross-contamination caused by previous extractions it is strongly recommended to note the sequence of the extractions.The following sample preparation should be applied for all kinds of samples:1. To maximize homogeneity and representativeness of the sample drawing, a minimum of 5g sample should be pulverized finely in a mortar, impact mill etc.2. 1g of the homogenized mixture is suspended in 20ml of pre-diluted extraction buffer. Afterwards the suspension is incubated for 15 min in a preheated water bath at 60°C. To ensure good homogeneity, the samples should be shaken every two minutes.3. The samples are centrifuged for 10 minutes at 2000xg. If it is not possible to separate the supernatant from the precipitate completely, the suspension should be filtered if necessary.4. 100ul of particle-free solution are applied per well. If the results of a sample are out of the measuring range, further dilution with the pre-diluted extraction and sample dilution buffer is necessary. The additional dilution has to be considered when calculating the concentration.Procedure:1. Prepare samples as described above. 2. Pipet 100ul ready-to-use standards or prepared samples in duplicate into the appropriate wells of the microtiter plate.3. Incubate for 20 minutes at room temperature. 4. Wash the plate three times as follows: Discard the contents of the wells (dump or aspirate). Pipet 300ul of diluted washing solution into each well. After the third repetition empty the wells again and remove residual liquid by striking the plate against a paper towel. The wash procedure is critical. Insufficient washing will result in poor precision and falsely elevated absorbencies.5. Pipet 100ul of conjugate (anti-ovomucoid IgG-HRP) into each well. 6. Incubate for 20 minutes at room temperature. 7. Wash the plate as outlined in step 4. 8. Pipet 100ul of substrate solution into each well.9. Allow the reaction to develop in the dark (e.g. cupboard or drawer; the chromogen is light-sensitive) for 20 minutes at room temperature.10. Stop enzyme reaction by adding 100ul of stop solution (0.5 M H2SO4) into each well. The blue color will turn yellow upon addition.11. After thorough mixing, measure absorbance at 450 nm (reference wavelength 620 nm), using an ELISA reader. The color is stable for 30 minutes.
- 产品特点
- 针对Egg White Residue, BioAssay ELISA Kit (Gallus gallus)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Egg White Residue, BioAssay ELISA Kit (Gallus gallus)产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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