- 中文名称
p53, BioAssay ELISA Kit (Human)
- 英文名字
- p53, BioAssay ELISA Kit (Human)
- 供应商
- USBiological
- 产品货号
- 301261
- 产品报价
- ￥1/100Tests
- 产品说明书
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- 购买方式
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- This p53 Enzyme-Linked Immunosorbent Assay (ELISA) BioAssay Kit is an in vitro assay for the quantitative measurement of native and recombinant human p53. The p53 protein is encoded by the TP53 gene. It is composed of 393aa but its apparent molecular mass is 53kD. p53 binds to a DNA consensus sequence, the p53 response element, and regulates normal cell cycle events by activating transcription of genes involved either in progression through the cell cycle, or causing arrest in G1 when the genome is damaged. It acts as a pivotal suppressor of inappropriate cell proliferation. By initiating suppressive effects through induction of apoptosis, cell senescence, or transient cell-cycle arrest, p53 plays an important role in cancer suppression, developmental regulation, and aging. p53 is found in increased amounts in a wide variety of transformed and tumor cells, where its concentration is increased 5-1000 fold over the concentration in normal cells. Meanwhile, p53 is also frequently mutated or inactivated in about 60% of cancers. Sample Type:Cell lysate and cell culture supernatant. Serum and plasma. Intended Use:Quantitative measurement of native and recombinant p53.Sensitivity:0.1ng/mlRange:0.2-25.6ng/mlSpecificity:Recognizes p53. Species Crossreactivity: human. This ELISA kit shows no cross-reactivity with other relevant proteins.Test Principle:The p53 ELISA BioAssay employs an antibody specific for human p53 coated on a 96-well plate. Standards and samples are pipetted into wells and p53 present in the sample is bound to the wells by immobilized antibody. Wells are washed and a biotin-labeled human p53 specific detection antibody is added. After washing away unbound detection antibody, a streptavidin HRP-conjugate is added to the wells. The wells are again washed, a TMB substrate solution is added, and color develops in proportion to the amount of bound p53. Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450nm. Recovery within this range is 86.3%. The intra-assay reproducibility as measured by the coefficient of variation (CV) is <8% and inter-assay has CV <12%.Kit Components:Plate Coated with p53 Ab, 1x96 wellsAssay Diluent, 1x27ml Wash Buffer A, 10x, 1x10ml Wash Buffer B, 10x, 1x10ml Wash Buffer C, 10x, 1x10ml rh p53 Standard, 3x vials Detection Antibody, 100X, 1x110ul Streptavidin-HRP Conjugate, 100X, 1x120ul TMB Substrate, 1x11ml Stop Solution, 1x11mlPlate Sealer, 2x sealers Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对p53, BioAssay ELISA Kit (Human)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到p53, BioAssay ELISA Kit (Human)产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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