- 中文名称
Nicotinamide-N-Methyltransferase Inhibitor Screening BioAssay Kit (NNMT)
- 英文名字
- Nicotinamide-N-Methyltransferase Inhibitor Screening BioAssay Kit (NNMT)
- 供应商
- USBiological
- 产品货号
- 301251
- 产品报价
- ￥1/100Tests
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- N′-Nicotinamide Methyltransferase (NNMT) (E.C. 2.1.1.1) catalyzes the N-methylation of nicotinamide, pyridines, and other analogues using S-adenosyl methionine (SAM) as the donor resulting in the production of 1-methylnicotinamide (MNA). NNMT plays a significant role in the regulation of metabolic pathways and is expressed at markedly high levels in several kinds of cancers, neurodegenerative diseases, obesity and diabetes, indicating it is a potential molecular target for therapy. Intended Use:For screening, studying, and characterizing potential inhibitors of NNMT.Specificity:Species Crossreactivity: mammalianTest Principle:This NNMT Inhibitor Screening BioAssay Kit utilizes SAM as the methyl group donor and nicotinamide as the substrate. NNMT methylates nicotinamide generating S-adenosylhomocysteine (SAH) and 1-methylnicotinamide. The SAH is hydrolyzed by SAH hydrolase to form homocysteine, the free thiol group of which is detected using a Thiol Detecting Probe generating enhanced fluorescence signal that can be measured at Ex/Em=392/482nm. In the presence of an NNMT inhibitor, the enzymatic activity is inhibited resulting in decreased fluorescence. This assay kit is a simple, sensitive, and rapid tool to screen potential inhibitors of NNMT.Kit Components:NNMT Assay Buffer, 1x22ml
NNMT Enzyme, 1x50ul
S-Adenosylmethionine (SAM), lyophilized, 4x vial
Nicotinamide, 3x1.5ml
Enzyme-I, 1x200ul
Enzyme-II, lyophilized, 1x vial
1-Methylnicotinamide (MNA), 150mM, 1x20ul
Thiol Detecting Probe, DMSO, 1x200ul
SAM Reconstitution Buffer, 1x500ulStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对Nicotinamide-N-Methyltransferase Inhibitor Screening BioAssay Kit (NNMT)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Nicotinamide-N-Methyltransferase Inhibitor Screening BioAssay Kit (NNMT)产品后将其置于-70°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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