c-Jun is a component of the transcription factor AP-1 that binds and activates transcription at TRE/AP-1 elements. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73. Extracellular signals including growth factors，transforming oncoproteins，and UV irradiation stimulate phosphorylation of c-Jun at Ser63/73 and activate c-Jun dependent transcription. The MAP kinase homologue，JNK，binds to the N-terminal region of c-Jun and phosphorylates c-Jun at Ser63/73. The activity of JNK is stimulated by the same signals that activate c-Jun.
应用类型
The antibody can be used in Western Blot analysis （0.5-4 ug/ml）. However， the optimal conditions should be determined individually. Recombinant human c-Jun （Cat.# 7001-100） can be used as a positive control.
免疫原
E. coli-expressed recombinant human c-Jun fusion protein
来源宿主
兔
反应性
经检测验证，可与人种属来源的c-Jun蛋白发生免疫反应
保存建议
提供的100 ug c-Jun抗体产品为无色溶液其浓度为0.5 mg/ml溶解于包含有30%甘油， 0.5% BSA， 5 mM EDTA和0.01%硫柳汞的PBS （pH 7.2）缓冲液中.请将该c-Jun抗体产品置于-20 °C冷冻保存，建议分装使用以避免反复冻融.
