- 中文名称
Optimax First Strand cDNA Synthesis BioGenomics Kit
- 英文名字
- Optimax First Strand cDNA Synthesis BioGenomics Kit
- 供应商
- USBiological
- 产品货号
- 299025
- 产品报价
- ￥1/100Tests
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- First Strand cDNA Synthesis BioGenomics Kit is developed based on cDNA synthesis protocol. The kit generates full-length first strand cDNA that is ready for PCR amplification. An easy to follow protocol is provided. The kit contains sufficient reagents for 100 cDNA synthesis reactions.Intended Use:Immediate PCR amplification of known genesComparison of gene expression patterns between biological samplesGene mutation analysisGene cloning and target sequencingTest Principle:This First Strand cDNA Synthesis BioGenomics Kit provides all necessary components to perform 100 first strand cDNA synthesis reactions from total RNA or poly(A) RNA. The system uses Moloney Murine Leukemia Virus Reverse Transcriptase with weak activity of RNase H that allows the synthesis of full-length cDNA from long templates. Oligo(dT)18 and random hexamer primers are provided. Specific primers can also be used with the kit.The cDNA synthesized using this system can be used directly in PCR amplification. The kit is optimized for maximum yield of full-length cDNA. The addition of ribonuclease inhibitor lowers the risk of mRNA degradation during the reaction.Quality Control:All kit components are DNase-, RNase-, and protease-free. Each component has been tested for purity and efficacy in first strand cDNA synthesis.Kit Components:MMLV reverse transcriptase, 100unit/ul, 1x100ulReaction Buffer, 5x, 1x1mlOligo(dT)18, 0.5ug/ul, 1x100ulRandom Hexamers, 0.2ug/ul, 1x100ulRNasein, 10units/ul, 1x100uldNTP, 10mM each, 1x250ulHuman Placenta total RNA, 1.25ug/ul, 1x10ulUpstream Primer, 25um, 1x50ulDownstream Primer, 25um, 1x50ulDEPC H2O, 1x1.25mlStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对Optimax First Strand cDNA Synthesis BioGenomics Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Optimax First Strand cDNA Synthesis BioGenomics Kit产品后将其置于-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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