- 中文名称
Luciferase Reporter Gene BioAssay Kit
- 英文名字
- Luciferase Reporter Gene BioAssay Kit
- 供应商
- USBiological
- 产品货号
- 299021
- 产品报价
- ￥1/200Tests ￥1/500Tests
- 产品说明书
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- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Intended Use:Gene Regulation: gene expression level, characterization of promoter activity, modulation of gene expression by receptors, transcription factors and small molecules.Drug Discovery: high-throughput screen for gene modulators.Sensitivity:Detection of as little of 2fg luciferase and as few as 4 cells.Sample Type:Mammalian, yeast or E. coli cellsTest Principle:The Luciferase Reporter Gene BioAssay is based on the quantitation of luciferase expression in mammalian, yeast or E. coli cells, using luciferin and ATP as substrates. The reaction results in light production which can be conveniently measured on a luminometer.Reaction scheme:LuciferaseATP+D-luciferin+O2 oxyluciferin+AMP+PPi+CO2+lightThis bioluminescent reporter gene assay is extremely sensitive and is especially suitable for quantifying luciferase expression in recombinant cells. This ultra-sensitive, homogeneous cell-based assay only requires adding a single reagent to the cells and measuring the light intensity after a short incubation step (2 minutes). Assays can be performed in tubes, cuvettes or multi-well plates. All kit components are compatible with culture media and with all liquid handling systems. With an extended luminescence emission kinetics (half-life 40 min), the luciferase assays are especially suitable for high-throughput screening in 96-well, 384-well and 1536-well plates. In addition, the reagent provided in the kits has been formulated for maximum sensitivity, reproducibility and long shelf-life. Applications for this kit includegene regulation studies and high-throughput screening of gene modulators.Data Analysis:The light intensity (RLU) is directly proportional to the luciferase concentration. For dose-response studies, the data are plotted against compound concentration and the EC50 for gene up-regulator compound and IC50 for a gene down regulator compound can be determined by non-linear regression analysis using Prism or other data analysis tools.Kit Components:Reagent, solidAssay Buffer, 1x20mlStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对Luciferase Reporter Gene BioAssay Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Luciferase Reporter Gene BioAssay Kit产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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