- 中文名称
DNA Methylation Detection BioAssay Kit
- 英文名字
- DNA Methylation Detection BioAssay Kit
- 供应商
- USBiological
- 产品货号
- 299011
- 产品报价
- ￥1/50Tests
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Methylated DNA occurs naturally in prokaryotes and eukaryotes. In humans, it has been implicated in epigenetics, genomic imprinting, embryonic development, X-chromosome inactivation and carcinogenesis. In humans, a majority of all cytosines in the dinucleotide 5’-CpG-3’ are methylated, though non-CpG methylation patterns have also been found. A breakthrough in the detection of DNA methylation was made with the discovery that bisulfite has higher specificity for cytosine than 5-methyl cytosine. The DNA after conversion is single stranded and non-complementary.Intended Use:This Genomic DNA Methylation Detection BioAssay Kit is developed and optimized for sodium bisulfite treatment.Range:2ug-500pgTest Principle:This DNA methylation detection kit offers a fast, efficient and convenient alternative to traditionalbisulfite conversions. This kit provides users with a true endogenous human positive control which eliminates any ambiguity in validating results. The positive control has been tested in over 15 different human tissues . The protocol is optimized to minimize DNA degradation, reagent preparation, and conversion time. The protocol guarantees ~99% conversion of cytosine to uracil and ~99% CpG protection, as calculated by sequencing, and is sensitive over a range of 2ug-500pg of DNA. In <3 hours, users can obtain high quality bisulfite modified DNA ready for analysis by PCR, sequencing, or microarrays. The kit is enough for 50 reactions.Kit Components:Conversion Reagent (CVR)*, 5x0.4g, RTActivator Solution (ACS)*, 1x5ml, RTStabilizer Solution (STS)*, 1x500ul, RTEquilibration Buffer (EQB), 1x20ml, RTDesulfonation Buffer (DSB), 1x4ml, RT Elution Buffer (TE), 2x1ml, RT Control MetPrimers (CMP), 5uM, 1x100ul, -20ºCControl Human DNA, 2ug, 1x100ul, -20ºCColumn 50x, RT*After mixing ACS, STS, and CVR to prepare Conversion Buffer (CVB), store at -20ºC.Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对DNA Methylation Detection BioAssay Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到DNA Methylation Detection BioAssay Kit产品后将其置于RT/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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